Presentation
The CAE team deploy a number of instruments dedicated to in situ measurements of bio-aerosols (pollens, mold, bacteria). This includes samplers for later analyses in the laboratory, and instruments for on-line real-time analyses.
Contact : Roland Sarda-Estève
Pollen and mold collector Lanzoni VPPS 2000
The Lanzoni type VPPS 2000 device is a daily volumetric sampler for collecting pollens and mold by impaction at 10 L per minute on a tansparent cellophane strip coated with silicone. Samples are collected weekly and analysed by optical microscopy.
Bacteria collecteur Leckel 47/50
The sequential sampler Leckel 47/50 is a filtration system operating at 33,4 L per minute, providing a daily sample on a 8-µm porosity polycarbonate membrane filter. The daily filters are collected weekly and particles are extracted in an aqueous medium. A first fraction is cultivated, a second one is analysed by chromatography, and a third one une troisième is used for genetic sequencing.
Pollen and mold real-time analyser Hund BAA500
The sampler and automated analyser Hund type BAA500 collects particules by impaction at 100 L per minute on a slide coated with a silicone gel. The samples are automatically analysed in 3 h by high-resolution optical microscopy associated with an artificial intelligence module for the identification of pollens and molds.
Pollen real-time analyser POLLENSENSE APS330
The sampler and automated analyser Pollensense type APS330 collects particules by impaction at 10 L per minute on a cellophane tape coated with silicone. The samples are continuously analysed by low-resolution optical microscopy associated with an artificial intelligence module for the identification of pollens. The results are available with a hourly time step.
Real-time analyser by holographic imagery SWISENS Jupiter Poleno
The automated pollen analyser Swisens type Jupiter Poleno is a high-resolution holographic imager. The particles are illuminated by a laser in the air flux of 40 L par minute. Particles are measured and analysed in real time thanks to an artificial intelligence module. Results are available with a time step of less than 1 hour.
Rapid chemistry for mold and bacteria analysis
Bio-aerosol collection by impaction and real-time analyses of chemical tracers of pollens, mold and bacteria in aqueous phase followed by a direct injection analysis by ion-exchange chromatography. The time resolution is 30 minutes. The analytical chain includes a Particle Into Liquid Sample (PILS, Metrohm) and a High-Performance Anion-Exchange Chromatography (HPAEC-PAD, Thermo scientific), operating at 16,7 L per minute.
Detection and real-time analysis of the fluorescence of single particles (0.5 to 20 µm in size) in the air flux, induced in different channels by pulsed halogen lamps. Particles are sized using a laser diode and side scattering measurement. The time resolution is better than 1 minute. The model used is a WIBS-4A from Droplet Measurement Technologies (DMT).
Resultats from intenive field campaigns allow fluorescence signals in the different channels to be deconvolved as a function of particle size, thanks to the assignment of characteristic chemical tracers obtained with a rapid chemistry analytical chain.
